The Comparator System for Colorimetric Chemical Analysis

The Comparator System is based on the following system components:

1. Comparator instrument to bring the treated sample and the glass filters together for color matching

2. Lighting unit (optional) to ensure constant viewing conditions for color matching

3. Color grading disc containing a series of glass filters which have been graded to give direct concentration values

4. Sample container of the required path length

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Using the Comparator System 2000

1. Follow the test method for sample preparation, adding the specified reagent to a known volume of sample in the appropriate sample container

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2. Insert the test disc into the comparator instrument

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3. Place the sample container in the right hand comparator compartment, and, if specified in the test method, a cell containing a blank of water or untreated sample in the left hand side

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4. Using standardized lighting, rotate the disc until the closest color match with the sample is obtained

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5. The value will be shown in the window in the comparator instrument.

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Medical & Clinical Tests

1. Select the appropriate test from the list below

2. Select the disc(s) with the required measuring range

3. In addition to the disc(s), it is necessary to use the comparator instrument, reagent and glassware as listed

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Test Disc Code Range Covered Chemical Method Based On Comparator Required Accessories Required Remarks
Alpha Amylase
Alpha Amylase In Blood or Urine 5/46 25, 100, 150, 175, 200, 225, 250, 300, 350 Somogyi units/100ml Starch and iodline Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of acute pancreatitis (damage to pancras)
Bilirubin
Bilirubin 5/3 0.2, 0.4, 0.6, 0.8, 1.0, 1.25, 1.5, 1.75, 2.0 mg/100ml Sulphanillic acid Comparator 2000+ 10ml, 13.5 mm cells Van den Bergh's method
Icterus Index 5/4 1, 3, 5, 7, 9, 11, 13, 15, 17 Icterus Index Comparison against serum Comparator 2000+ 10ml, 13.5 mm cells Meulengracht's method
Serum Bilirubin 5/42 1, 2, 3, 4, 5, 6, 7, 8, 9mg/100ml Lathe & Ruthven Comparator 2000+ 10ml, 13.5 mm cells Direct and total pigment
Cholesterol
Cholesterol 5/15 80, 120, 160, 200, 250, 300, 350, 400, 500mg/100ml Acetic anhydride Comparator 2000+ Stoppered tubes AF224 Diagnosis of cholesterol levels
Cholesterol 5/45 150, 200, 250, 300, 350, 400, 450, 500, 550mg/100ml Ferric chloride/sulphuric acid Comparator 2000+ 10 ml, 13.5mm cells @
Cholinesterase
Cholinesterase, Activity In Blood 5/30 0, 12.5, 25, 37.5, 50, 62.5, 75, 87.5, 100% Bromothymol blue and substrate Comparator 2000+ 2.5 mm cells W680/OG/2.5 Rapid testing of exposure to organo phosphorus insecticide. Test kit, AF 267
Habmoglobin Levels In Blood, Hbmoglobin Count
Habmoglobin 20-58% 5/8A 20, 24, 28,32, 36, 40, 46, 52, 58% Direct colour of blood Comparator 2000+ 0.004" blood cell. W670 Harrison's method Test Kit AF353
Habmoglobin 64-130% 5/8B 64, 70, 76, 84, 92, 100, 110, 120, 130% Direct colour of blood Comparator 2000+ 0.004" blood cell W670 Harrison's method Test Kit AF353
Habmoblobin 8-16 5/37 8, 9, 10, 11, 12, 13, 14, 14.6 16g/100ml Oxyhaemoglobin Comparator 2000+ 10ml, 13.5 mm cells @
Habmoglobin 3-14 5/37X 3, 4, 5, 6, 7, 8, 10, 12, 14g/100ml Oxyhaemoglobin Comparator 2000+ 10ml, 13.5 mm cells @
Habmoglobin 4-16 5/37Z 4, 5.5, 7, 8.5, 10, 11.5, 13, 14.5, 16g/100ml Oxyhaemoglobin Comparator 2000+ 10ml, 13.5 mm cells @
Iron
Iron in Serum 5/50 25, 50, 75, 100, 125, 150, 175, 200, 225 micrograms/100ml Bathophenanthroline Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of anaemias, hepatitis
Lactate
Lactate Dehydrogenase 5/56 83, 167, 250, 333, 416, 500, 583, 667, 882 Lactate and 2:4 dinitrophylhydrazin Comparator 2000+ 10ml, 13.5mm cells Diagnosis of heart and liver disorders
Lead
Lead In urine/Faeces 5/17 2, 4, 6, 8, 10, 12, 14, 16, 20 micrograms Dithizone Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of lead contamination
pH Value of Blood/Urine
pH of blood 7-7.4 5/18A 7.0, 7.05, 7.1, 7.15, 7.2, 7.25, 7.3, 7.35, 7.4 Saline phenol red Comparator 2000+ 10ml, 13.5 mm cells
pH of blood 7.4-7.8 5/18B 7.4, 7.45, 7.5, 7.55, 7.6, 7.65, 7.7, 7.75, 7.8 Saline phenol red Comparator 2000+ 10ml, 13.5 mm cells
pH of urine 4-11 2/1p 4, 5, 6, 7, 8, 9, 10, 11 Universal Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of alkalaemia/acidaemia
Phenolsulphophthalein
Phenolsulphophthalein (Phenol Red) Excretion test 5/44 5, 10, 15, 20, 25, 30, 35, 40, 45% Phenolsulphonphthalein Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of renal (kidney) efficiency
Phenylpyruvic
Phenylpyruvic Acid 5/6 20,  40, 50, 60, 70, 80, 90, 100 mg/100ml 2:4 dinitrophenyl hyfrazine Comparator 2000+ 5 mm cell W680/OG/5 In urine
Phosphatase In Blood
Alkaline/Acid 5/122 8, 12, 16, 20, 25, 30, 40, 50, 60 KA units Phosphotungstic-phosphophpmplybdic acid Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of certain disorders of bone, liver disease
Alkaline/Acid 5/53 Alkaline 2, 4, 6, 8, 10, 2, 14, 16, 18 KA units (Acid 1, 2, 3, 4, 5, 6, 7, 8, 9 KA units) 4 amino antopyrene/pot ferrivyanide Comparator 2000+ 10ml, 13.5mm cells Diagnosis of certain disorders of bone, liver disease
Formol Stable Acid 5/55 1, 2, 3, 4, 5, 6, 7, 8, 9KA units 4 amino antopyrene/pot ferricyanide Comparator 2000+ 10ml, 13.5mm cells Diagnosis of carcinoma of the prostate
Phosphorus
Phosphorus Inorganic, In Blood 5/14 0.02, 0.03, 0.04, 0.05, 0.06, 0.08, 0.1, 0.12, 0.16 mg P in 1 ml blood Amino naphthol sulphonic acid Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of certain bone disorders, especially rickets; diseases of the parathyroid glands
Proteins
Proteins 20-180 5/5A 20, 40, 60, 80, 100, 120, 140, 160, 180mg/100ml Copper sulphate (Buiret method) Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of liver disease
Proteins 200-340 5/5B 200, 220, 240, 260, 280, 300, 320, 340, 360mg/100ml Copper sulphate (Buiret method) Comparator 2000+ 10ml, 13.5 mm cells
Serum Proteins 5/54A 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5mg/100ml Copper sulphate (Buiret method) Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of chronic infective and neoplastic conditions
Serum Proteins 5/54B 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5g/100ml Copper sulphate (Buiret method) Comparator 2000+ 10ml, 13.5 mm cells
Salicylate
Salicylate In Blood 5/51 15, 25, 35, 45, 55, 65, 80, 95, 110mg/100ml Ferric nitrate Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of salicylate 'Asoirin' poisoning
Sugar/Glucose In Blood
Sugar In Blood 5/2A 60, 80, 100, 120, 140, 160, 180, 200, 220mg/100ml Phosphomolybdic acid Comparator 2000+ Folin tube AF 280, 10ml, 13.5mm cells Total reducing substances in blood
Sugar In Blood 5/2B 240, 260, 280, 300, 320, 340, 360, 380, 400mg/100ml Phosphomolybdic acid Comparator 2000+ Folin tube AF 280, 10ml, 13.5 mm cells Total reducing substances in blood
Sugar In Blood 5/2C 20, 30, 40, 50, 60, 70, 80, 90, 100mg/100ml Phosphomolybdic acid Comparator 2000+ Folin tube AF 280, 10ml, 13.5 mm cells Total reducing substances in cerebrospinal fluid and other body fluids
Sugar In Blood 5/36A 40, 60, 80, 100, 120, 140, 160, 180, 200mg/100ml Phosphomolybdic asid Comparator 2000+ 10ml, 13.5 mm cells Harding's Method for true sugar values
Sugar In Blood 5/36B 220, 240, 260, 280, 300, 320, 340, 370, 400mg/100ml Phosphomolybdic acid Comparator 2000+ 10ml, 13.5 mm cells Harding's Method for true sugar values
Glucose In Blood 5/48 50, 75, 100, 125, 150, 175, 200, 250mg/100ml 4 amino phenozone Comparator 2000+ 10ml, 13.5 mm cells True glucose value in blood
Sulphobromophthalein
Sulphobromophthalein Retention 5/32 0, 5, 10, 15, 20, 25, 30, 40, 50% 10ml, 13.5 mm cells Diagnosis of liver disease
Sulphonamides
Sulphonamides In Blood/Urine 5/10A 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0, 7.0, 8.0mg/100ml Diazotisation Comparator 2000+ 10ml, 13.5 mm cells Therapeutic response of sulphonamides in treeating meningococcal infections
Trichloro-Acetic Acid
Trichloro-Acetic Acid 5/31 10, 20, 30, 40, 50, 75, 100, 150, 200 ppm P Pyridine Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of exposure to trichlorethylene solvents
Serum Plasma
Serum/Plasma Urea 5/43 20, 40, 60, 80, 100, 120, 140, 160, 180mg/100ml Diacetyl monoxime Comparator 2000+ 10ml, 13.5 mm cells
Serum/Plasma Urea 5/9A 20, 30, 40, 50 , 60, 70, 80, 90, 100mg/100ml Neessler's Reagent/Urease Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of kidney disorder and other conditions
Serum/Plasma Urea 5/9B 110, 120, 130, 140, 150, 160, 180, 200, 220mg/100ml Nessleer's Reagent/Urease Comparator 2000+ 10ml,13.5 mm cells
Uric Acid
Uric Acid In Serum 5/47 1, 2, 3, 4, 5, 6, 7, 8, 9mg/100ml Alkaline phossphotungstate Comparator 2000+ 10ml, 13.5 mm cells Diagnosis of kidney disorder, gout, leukaemia and other conditions

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